Analytical chemistry

Functional protein analysis often calls for lengthy, laborious in vivo protein expression and purification, and can be complicated by the lack of stability of the purified protein. In this study, we demonstrate the feasibility of a simplified procedure for functional protein analysis on magnetic particles using cell-free protein...

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN...

A new liquid chromatography (LC)-negative ion electrospray ionization (ESI)–tandem mass spectrometry (MS/MS) method with post-column addition of ammonia in methanol has been developed for the analysis of acid herbicides: 2,4-dichlorophenoxy ace- tic acid, 4-chloro-o-tolyloxyacetic acid, 2-(2-methyl-4-chlorophenoxy)butyric acid, mecoprop, dichlorprop, 4-(2,4-dichlorophenoxy) butyric acid, 2,4,5-trichlorophenoxy propionic acid, dicamba and bromoxynil, along with...

A Matlab implemented computer code for spectral resolution is presented. The code enables the user to resolve the UV-visible absorption spectrum of a mixture of up to 3 previously known components, to the individual components, thus, evaluating their quantities. The resolving procedure is based on searching the combination of the...

The present study employs time of flight mass spectrometry for quantitative analysis of the local anesthetic drugs ropivacaine and bupivacaine in authentic, pharmaceutical and spiked human plasma as well as in the presence of their impurities 2,6-dimethylaniline and alkaline degradation product. The method is based on time of flight electron...

Two sensitive and validated methods were developed for determination of a racemic mixture citalopram and its enantiomer S-(+) escitalopram. The first method was based on direct measurement of the intrinsic fluorescence of escitalopram using sodium dodecyl sulfate as micelle enhancer. This was further applied to determine escitalopram in spiked human...

New simple spectrofluorimetric method with enhanced sensitivity has been developed and validated for the determination of the antidepressant paroxetine (PXT) in its dosage forms and plasma. The method was based on nucleophilic substitution reaction of PXT with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in an alkaline medium (pH 8) to form a highly fluorescent derivative...

The aim of this study is to adopt the approach of metabolic fingerprinting through the use of Fourier Transform Infrared (FTIR) technique to understand changes in the chemical structure in Padina tetrastromatica (Hauck). The marine brown alga under study was grown in two different environmental conditions; in natural seawater (P....

This work described study protocols on the production of Palm-Based Standard Reference Materials for iodine value and slip melting point. Thirty-three laboratories collaborated in the inter-laboratory proficiency tests for characterization of iodine value, while thirty-two laboratories for characterization of slip melting point. The iodine value and slip melting point of...

A comparison of detection limits of gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) with gas chromatography-tandem mass spectrometry (GC-MS/MS) in selected reaction monitoring (SRM) mode with both electron ionization (EI) and negative-ion chemical ionization (NCI) are presented for over 50 pesticides ranging from organochlorines (OCs), organophosphorus pesticides (OPs)...

Microalbuminuria is associated with hypertension and is a strong risk factor for subsequent chronic disease, both renal and coronary heart disease (CHD), Presently there are several methods available for measurement of microalbuminuria. The aim of this study was to evaluate if the three different methods gave similar information or if...

A simple high sensitive, selective, and rapid spectrophotometric method for the determination of trace gold based on the rapid reaction of gold(III) with bis(salicylaldehyde)orthophenylenediamine (BSOPD) in aqueous and micellar media has been developed. BSOPD reacts with gold(III) in slightly acidic solution to form a 1:1 brownish-yellow complex, which has an...

A simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of griseofulvin in small volumes of rat plasma was developed and validated using warfarin as an internal standard. Biological sample preparation involved simple extraction with acetonitrile, followed by dilution with aqueous mobile phase buffer (20...

Marcio L. Rodrigues1, Leonardo Nimrichter1, Debora L. Oliveira1, Joshua D. Nosanchuk2,3 and Arturo Casadevall2,3 1Laboratório de Estudos Integrados em Bioquímica Microbiana, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, 21941590, Brazil. 2Department of Microbiology and Immunology and the 3Division of Infectious Diseases of the...

Micellar electrokinetic chromatography (MEKC) was investigated for the simultaneous determination of letrozole, imipramine and their metabolites in human urine samples over a concentration range of therapeutic interest. Experimental parameters such as pH of the running electrolyte, sodium dodecylsulphate (SDS) concentration, borate concentration, voltage, etc were investigated. Under optimal conditions of...

Michalakis Savva Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, Brooklyn, NY 11201. Abstract Using a Taylor expansion to first order, a novel method was developed to calculate the uncertainty of drug concentration in pharmaceutical dosage...

Alaa Khedr, Mohamed Moustafa, Ashraf B. Abdel-Naeim, Abdulrahman Alahdal, and Hisham Mosli Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia Abstract A validated high-performance liquid chromatographic (HPLC) method for determination of phenytoin (PHN), para-hydroxy metabolite of phenytoin (POH) and sildenafil (SIL) in rabbit plasma is described. The...

John Geraldine Sandana Mala1 and Satoru Takeuchi2 1SANDANA FLORALS, Module-7, Golden Jubilee Biotech Park for Women Society, In SIPCOT-IT Park, Old Mahabalipuram Road, Siruseri, Navalur P.O., Kanchipuram District-603103, Tamilnadu, India. 2Factory of Takeuchi Nenshi, TAKENEN, 85 NE, Takamatsu, Kahoku, Ishikawa 929-1215, Japan. Abstract The structural elucidations of microbial...

Tadashi Yoshida1, Akira Honda2, Hiroshi Miyazaki3 and Yasushi Matsuzaki2 1Raffles Japanese Clinic, Singapore, Singapore. 2Tokyo Medical University, Kasumigaura Hospital, Ibaraki, Japan. 3Pharmax Institute, Kanagawa, Japan. Abstract For more than a decade, we have developed stable isotope dilution mass spectrometry methods to quantify key intermediates in cholesterol and bile...

Herrero MJ1, Botella R2, Algás R3, Marco FM4 and Aliño SF1 1Gene Therapy Group, Dpto. Farmacologia, Fac. Medicina, Univ.Valencia, Valencia, España. 2Servicio Dermatologia, Instituto Valenciano Oncologia,Valencia, España. 3Servicio Radioterapia, Hospital Clínico Universitario,Valencia, España. 4ASAC Pharmaceutical International, Alicante, España. Abstract Cancer vaccines have always...

Gene associated with retinoid-interferon-β-induced mortality (GRIM)—19, was originally identifi ed as a critical regulatory protein necessary for Interferon-β-Retinoic acid-induced cell death. Overexpression of GRIM-19 activates cell death and its suppression or inactivation promotes cell growth. GRIM-19 targets multiple proteins/pathways for exerting growth control and cell death. However, GRIM-19 is also...

Nafisur Rahman and Sk Manirul Haque Department of Chemistry, Aligarh Muslim University, Aligarh (U.P.) 202 002, India. Abstract Four simple, rapid and sensitive spectrophotometric methods have been proposed for the determination of enalapril maleate in pharmaceutical formulations. The first method is based on the reaction of carboxylic acid...

Medicharla V. Jagannadham and Ramakrishnan Nagaraj Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India. Abstract Phosphopeptides with one and four phosphate groups were characterized by MALDI mass spectrometry. The molecular ion of monophosphopeptide could be detected both as positive and negative ions by MALDI...

Helena Gonzalez1, Carl-Eric Jacobson2, Ann-Marie Wennberg1, Olle Larkö1 and Anne Farbrot2,3 1Department of Dermatology and Venereology, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. 2Department of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. 3 Current address SCA. Abstract Background: Benzophenone-3 (BZ-3) is a common ultraviolet...

Fatma Al-Saeedi Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Kuwait. Abstract: Pentavalent technetium-99m dimercaptosuccinic acid (99mTc-(V)DMSA) is a tumor-seeking agent which was introduced to evaluate, image, and manage many types of cancers. In this review, the beginning of, and the most...

S.J. Shahtaheri1, M. Khadem1, F. Golbabaei1 and A. Rahimi-Froushani2 1Department of Occupational Health, Tehran University of Medical Sciences, Iran. 2Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences, Iran.Abstract: Chromium is an important constituent widely used in different industrial processes for production...

Xing-Zheng Wu1, Tomohisa Kato1, Yumiko Tsuji1 and Satoshi Terada2 1Department of Materials Science and Engineering, 2Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui-Shi 910-8507, Japan. Abstract: This research shows a novel method for hazard identification of a chemical and UV...

Eiichi Saitoh1, Shinya Yamamoto1, Eishiro Okamoto1, Yoshimi Hayakawa1, Takashi Hoshino1, Ritsuko Sato2, Satoko Isemura2, Sadami Ohtsubo3 and Masayuki Taniguchi4 1Graduate School of Technology, Niigata Institute of Technology, Kashiwazaki, Niigata 945-1195, Japan.2The Nippon Dental University College at Niigata, Niigata, Niigata 951-8580, Japan.3Food Research Center, Niigata Agricultural Research Institute, Kamo, Niigata 959-1381.4Department...

Chandrika J Piyathilake, Constance B Robinson and Phillip Cornwell Department of Nutrition Sciences, University of Alabama at Birmingham, AL 35294.Abstract: The measurement of folate in red blood cells (RBCs) is preferred since it reflects long-term folate status in the body compared to plasma/serum folate which may be influenced by recent...

Tomoko Niwa1, Tetsuo Asaki1 and Shinya Kimura2 1Discovery Research Laboratories, Nippon Shinyaku Co., Ltd. 14, Nishinosho-Monguchi-Cho, Kisshoin, Minami-ku, Kyoto 601-8550, Japan. 2Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 600-8507, Japan.Abstract: Protein kinases catalyze the transfer of the γ-phosphoryl group of adenosine triphosphate...