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Biochemistry Insights

Synopsis: An open access, peer reviewed electronic journal that covers biochemistry.


Indexing: Three major databases. Pubmed indexing for NIH-funded research.

Processing time: Decision in 2 weeks for 90% of papers.

Visibility: Most popular article read 800+ times.

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About this journal

ISSN: 1178-6264



Aims and scope:

Biochemistry Insights is a peer-reviewed, open-access online journal encompassing all topics within the field of biochemistry. This includes the chemistry, structure and functionality of proteins, carbohydrates, lipids, nucleic acids and smaller molecular components of cells and tissues. Cell metabolism, genomes, protein synthesis, cell signalling, transportation, and signal transduction are included but are not exclusive subjects. The journal also accepts articles on related topics applicable to this area, such as technology, outcomes, techniques and applications.

Editorial standards and procedures:

Submissions, excluding editorials, letters to the editor and dedications, will be peer reviewed by two reviewers.  Reviewers are required to provide fair, balanced and constructive reports.  

Under our Fairness in Peer Review Policy authors may appeal against reviewers' recommendations which are ill-founded, unobjective or unfair.  Appeals are considered by the Editor in Chief or Associate Editor.

Papers are not sent to peer reviewers following submission of a revised manuscript. Editorial decisions on re-submitted papers are based on the author's response to the initial peer review report.

Indexing:

This journal is indexed by:

  • CAS 
  • DOAJ
  • OAIster
National Institutes of Health Public Access Policy compliant:

As of April 7 2008, the US NIH Public Access Policy requires that all peer reviewed articles resulting from research carried out with NIH funding be deposited in the Pubmed Central archive.

If you are an NIH employee or grantee Libertas Academica will ensure that you comply with the policy by depositing your paper at Pubmed Central on your behalf. 



 
 
 


Prolonged Treatment with Free Fatty Acids has Post Receptor Effect in Hepatic Insulin Resistance: Evidence that Fatty Acids, Oleate and Palmitate have Insignificant Effect on the Insulin Receptor Beta In Vivo and Ex Vivo Primary Hepatocytes

Authors: Rafik Ragheb, Amina M. Medhat, Gamila M.L. Shanab, Dina M. Seoudi and Fantus I.G.
Publication Date: 12 Jun 2009
Biochemistry Insights 2009:2 29-36

Rafik Ragheb1,2,3,4,5, Amina M. Medhat1, Gamila M.L. Shanab1, Dina M. Seoudi1 and Fantus I.G.2,3,5

1University of Ain Shams, Department of Biochemistry, Faculty of Science, Cairo, Egypt. 2University Health Network, 3Mount Sinai Hospital, 4Hospital for Sick Children, 5University of Toronto, Toronto, Canada.   

Abstract

In the current study, we used immunoprecipitation and immunoblotting to examine the levels and phosphorylation status of the insulin receptor-beta subunit (IR-β), as well as the down stream target in PI3K pathway, total PKB/Akt as well as their phosphorylated forms. The assessment of FFAs treatment showed no direct and significant effect on the PI3K stimulation, specifically the IR-β in primary hepatic control cells treated with insulin. Cells treated with either oleate or palmitate (360 µM) showed no statistically significant values following insulin stimulation (P > 0.05). To further investigate the effect of both FFAs and high insulin (1 µg), we examined the effects of oleate and palmitate at 360 µM concentration on IR-β as well as PKB. There was no significant difference in the total protein levels and their phosphorylated forms in cells treated with or without oleate or plamitate. Interestingly, IR-β tyrosine phosphorylation showed a similar insignificant effect in vivo and ex vivo hepatic cells treated with oleate or palmitate in comparison to their controls in the fructose fed hamsters.

Categories: Biochemistry


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