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Biochemistry Insights

Synopsis: An open access, peer reviewed electronic journal that covers biochemistry.


Indexing: Three major databases. Pubmed indexing for NIH-funded research.

Processing time: Decision in 2 weeks for 90% of papers.

Visibility: Most popular article read 800+ times.

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About this journal

ISSN: 1178-6264


Aims and scope:

Biochemistry Insights is a peer-reviewed, open-access online journal encompassing all topics within the field of biochemistry. This includes the chemistry, structure and functionality of proteins, carbohydrates, lipids, nucleic acids and smaller molecular components of cells and tissues. Cell metabolism, genomes, protein synthesis, cell signalling, transportation, and signal transduction are included but are not exclusive subjects. The journal also accepts articles on related topics applicable to this area, such as technology, outcomes, techniques and applications.

Editorial standards and procedures:

Submissions, excluding editorials, letters to the editor and dedications, will be peer reviewed by two reviewers.  Reviewers are required to provide fair, balanced and constructive reports.  

Under our Fairness in Peer Review Policy authors may appeal against reviewers' recommendations which are ill-founded, unobjective or unfair.  Appeals are considered by the Editor in Chief or Associate Editor.

Papers are not sent to peer reviewers following submission of a revised manuscript. Editorial decisions on re-submitted papers are based on the author's response to the initial peer review report.

Indexing:

This journal is indexed by:

  • CAS 
  • DOAJ
  • OAIster
National Institutes of Health Public Access Policy compliant:

As of April 7 2008, the US NIH Public Access Policy requires that all peer reviewed articles resulting from research carried out with NIH funding be deposited in the Pubmed Central archive.

If you are an NIH employee or grantee Libertas Academica will ensure that you comply with the policy by depositing your paper at Pubmed Central on your behalf. 



 
 
 


Use of a Combined Duplex PCR/Dot Blot Assay for more sensitive genetic characterization

Authors: Erin Curry, Scott L. Pratt, Dale E. Kelley, Daniel R. Lapin and John R. Gibbons
Publication Date: 06 Nov 2008
Biochemistry Insights 2008:1 35-39

Erin Curry1, Scott L. Pratt1, Dale E. Kelley1, Daniel R. Lapin2 and John R. Gibbons1,2

1Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC 29634. 2OvaMax, Inc. Watertown, WI 53094.

Abstract

A reliable and sensitive method of genetic analysis is necessary to detect multiple specific nucleic acid sequences from samples containing limited template. The most widely utilized method of specific gene detection, polymerase chain reaction (PCR), imparts inconsistent results when assessing samples with restricted template, especially in a multiplex reaction when copies of target genes are unequal. This study aimed to compare two methods of PCR product analysis, fluorescent detection following agarose gel electrophoresis or dot blot hybridization with chemiluminescent evaluation, in the detection of a single copy gene (SRY) and a multicopy gene (β-actin). Bovine embryo sex determination was employed to exploit the limited DNA template available and the target genes of unequal copies. Primers were used either independently or together in a duplex reaction with purified bovine genomic DNA or DNA isolated from embryos. When used independently, SRY and β-actin products were detected on a gel at the equivalent of 4-cell or 1-cell of DNA, respectively; however, the duplex reaction produced visible SRY bands at the 256 cell DNA equivalent and β-actin products at the 64 cell DNA equivalent. Upon blotting and hybridization of the duplex PCR reaction, product was visible at the 1–4 cell DNA equivalent. Duplex PCR was also conducted on 186 bovine embryos and product was subjected to gel electrophoresis or dot-blot hybridization in duplicate. Using PCR alone, sex determination was not possible for 22.6% of the samples. Using PCR combined with dot blot hybridization, 100.0% of the samples exhibited either both the male specific and β-actin products or the β-actin signal alone, indicating that the reaction worked in all samples. This study demonstrated that PCR amplification followed by dot blot hybridization provided more conclusive results in the evaluation of samples with low DNA concentrations and target genes of unequal copies.

Categories: Biochemistry


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