Drug Target Insights
Synopsis: An open access, peer reviewed electronic journal that covers drug treatment targets.
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About this journal
Aims and scope:
Drug Target Insights covers current developments in all areas of the field of clinical therapeutics. The journal has two specific areas of focus:
- On molecular drug targets, including disease-specific proteins, receptors, enzymes, and genes.
- The journal seeks to elucidate the impact of new therapeutic agents on patient acceptability, preference, satisfaction and quality of life.
Drug Target Insights seeks to be the most up-to-date journal for those who need to be informed of the latest and most important developments in the field. The journal seeks to be the most reliable and up-to-date journal in this field by offering rapid and credible pre-production submission processing to authors. By publishing in open-access format, authors are able to communicate with the widest possible group of readers.
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Submissions, excluding editorials, letters to the editor and dedications, will be peer reviewed by two reviewers. Reviewers are required to provide fair, balanced and constructive reports.
Under our Fairness in Peer Review Policy authors may appeal against reviewers' recommendations which are ill-founded, unobjective or unfair. Appeals are considered by the Editor in Chief or Associate Editor.
Papers are not sent to peer reviewers following submission of a revised manuscript. Editorial decisions on re-submitted papers are based on the author's response to the initial peer review report.
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As of April 7 2008, the US NIH Public Access Policy requires that all peer reviewed articles resulting from research carried out with NIH funding be deposited in the Pubmed Central archive.
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The Way that PEGyl-DSPC Liposomal Doxorubicin Particles Penetrate into Solid Tumor Tissue
Xing Qing Pan1, Susie Jones2 and Karen Cox2
1College of Pharmacy, 2Department of Pathology, Medical Center. The Ohio State University, Columbus, OH 43210.
Abstract
Background: For enhancement of drug effectiveness and reduction of drug toxicity, liposomal drugs have been studied in laboratories and clinics for decades. Although the results obtained from in vitro are encouraging, but the results from in vivo tests were not satisfactory. The main reasons for this situation were that we do not have enough information about the way how liposomal particles penetrating into solid tumor tissue, and what happening to the liposome particles after they got into the tumor tissue. In this paper, we are going to report the results from our observations on the way folic acid targeted and non-targeted PEGyl-DSPC liposomal doxorubicin particles penetrate into solid tumor tissue.
Methods: Subcutaneous transplanted murine L1210JF solid tumors in mice were used as a model. PEGyl liposomal doxorubicins were injected through tail venue, and tumor tissue samples were collected at special time points. Cryosections were cut and dried by a fl owing of air after mounted on the slides right away. Then the dried cryosections were stained in water systems; the blood vessel cells were stained with green fluorescent FITC labeled antibody against CD31 antigen; the nuclei of the living cells were stained with a blue fluorescent dye DAPI. Since the whole procedure was carried out in aquatic system, the red color fluorescent liposomal doxorubicin particles remain visible under fl uorescence microscope.
Results: Both folate conjugated and non-conjugated PEGyl-DSPC liposomal doxorubicin particles were only leaking out from the broken holes of blood vessels with a special direction and spread out for a limited distance, which was similar to the results showed before, in that observation a latex microsphere sample was used as a model.
Abbreviations: DSPC:1, 2-Distearoyl-sn-Glycero-3-phosphatylcholine; PEG2000-DSPC:1, 2-Distearoyl-sn-Glycero 3-phosphatidylethanolamine-N-[methoxy(polyethylene glycol)-2000]; Folate-PEG3400-DSPE:1, 2-Distearoyl-sn-Glycero- 3-phosphatidylethanolamine-N-[polyethylene glycol-3400]-folate.
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