Wilfredo Resto¹, Joan Roque¹, Rosamil Rey^2,3, Héctor Colón², and José Zayas²

¹Department of Chemistry University of Puerto Rico-Cayey, Antonio R. Barceló Ave. Cayey P.R. 00736; ²Research and Development Division, ZAYCOR Industries Corporation, 828 de Diego Ave. Caparra Terrace, San Juan PR 00921; ³Interamerican University, Bayamón Campus, Department of Natural Sciences, Bayamon, Puerto Rico 00957.

Abstract: Anion chromatography with conductivity detection was chosen as the analytical technique for the development of a cleaning validation method for clean-in-place (CIP) detergents. The method was developed and validated for the determination of traces of the detergent CIP-200. It was shown to be linear with a squared correlation coefficient (r2) of 0.9999 and the accuracy experiments presented average recoveries of 88.2% (area response factor) from stainless steel surfaces. The repeatability was found to be 1.6% and an intermediate precision of 1.9% across the range. The method was also shown to be sensitive with an average Detection Limit (DL) of 0.23 ppm and a Quantitation Limit (QL) of 0.70 ppm based on the amount of phosphate in the detergent sample. The phosphate signal was well resolved from typical ions encountered in water samples or any other interference presented from swabs and surfaces. The method was applied to cleaning validation samples and proved to be suitable for rapid and reliable quality control.

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