Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata

Kei Shimoda; Naoji Kubota; Hatsuyuki Hamada; Tatsunari Kobayashi; Hiroki Hamada; Siddiqi M. Shafi; Nobuyoshi Nakajima

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Biochemistry Insights

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Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata

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Authors: Kei Shimoda, Naoji Kubota, Hatsuyuki Hamada, Tatsunari Kobayashi, Hiroki Hamada, Siddiqi M. Shafi and Nobuyoshi Nakajima

Publication Date: 06 Jan 2009

Type: Original Research

Journal: Biochemistry Insights

Citation: Biochemistry Insights 2009:2 5-7

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Abstract

Incubation of methyl 2-benzamidomethyl-3-oxobutanoate with cultured plant cells of Parthenocissus tricuspidata for 2 days afforded methyl (2R,3S)-2-benzamidomethyl-3-hydroxybutanoate with 100% de and 99% ee in 51% yield. The use of immobilized cells of P. tricuspidata in calcium alginate gel improved the yield of the product (95% yield) with 100% de and 99% ee. The immobilized cells of P. tricuspidata maintained the high potential for the reduction of methyl 2-benzamidomethyl-3-oxobutanoate (85% yield) after 5 times of usage.

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