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Microbiology Insights

Synopsis: An open access, peer reviewed electronic journal that covers microbiology.


Indexing: 4 major databases. Pubmed indexing for NIH-funded research.

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About this journal

ISSN: 1178-6361


Aims and scope:

Microbiology Insights is a peer-reviewed, open access journal which encompasses all aspects of the study of microbiology. Studies on all aspects of both prokaryotic and eukaryotic microorganisms and viruses are welcome. Areas such as functionality, infectious disease, food-borne pathogens, drug resistance and antimicrobials, industrial applications and more are also incorporated in this journal. Microorganisms are of increasing importance today, in terms of subjects of study as a way of understanding more complex organisms and processes, as an industrial and medical tool, and also a threat. Open access online journals are more accessible and publish rapidly, allowing greater dissemination of knowledge in this crucial field.

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Submissions, excluding editorials, letters to the editor and dedications, will be peer reviewed by two reviewers.  Reviewers are required to provide fair, balanced and constructive reports.  

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As of April 7 2008, the US NIH Public Access Policy requires that all peer reviewed articles resulting from research carried out with NIH funding be deposited in the Pubmed Central archive.

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Intestinal Spirochaetes of the Genus Brachyspira Share a Partially Conserved 26 Kilobase Genomic Region with Enterococcus faecalis and Escherichia coli

Authors: Yair Motro, David S. Dunn, Tom La, Nyree D. Phillips, David J. Hampson and Matthew I. Bellgard
Publication Date: 15 May 2008
Microbiology Insights 2008:1 3-11

Yair Motro1, David S. Dunn1, Tom La2, Nyree D. Phillips2, David J. Hampson2 and Matthew I. Bellgard1

1Centre for Comparative Genomics, Murdoch University, Murdoch, Western Australia, Australia. 2School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.

Abstract

Anaerobic intestinal spirochaetes of the genus Brachyspira include both pathogenic and commensal species. The two best-studied members are the pathogenic species B. hyodysenteriae (the aetiological agent of swine dysentery) and B. pilosicoli (a cause of intestinal spirochaetosis in humans and other species). Analysis of near-complete genome sequences of these two species identifi ed a highly conserved 26 kilobase (kb) region that was shared, against a background of otherwise very little sequence conservation between the two species. PCR amplification was used to identify sets of contiguous genes from this region in the related Brachyspira species B. intermedia, B. innocens, B. murdochii, B. alvinipulli, and B. aalborgi, and demonstrated the presence of at least part of this region in species from throughout the genus. Comparative genomic analysis with other sequenced bacterial species revealed that none of the completely sequenced spirochaete species from different genera contained this conserved cluster of coding sequences. In contrast, Enterococcus faecalis and Escherichia coli contained high gene cluster conservation across the 26 kb region, against an expected background of little sequence conservation between these phylogenetically distinct species. The conserved region in B. hyodysenteriae contained five genes predicted to be associated with amino acid transport and metabolism, four with energy production and conversion, two with nucleotide transport and metabolism, one with ion transport and metabolism, and four with poorly characterised or uncertain function, including an ankyrin repeat unit at the 5’ end. The most likely explanation for the presence of this 26 kb region in the Brachyspira species and in two unrelated enteric bacterial species is that the region has been involved in horizontal gene transfer.

Categories: Microbiology


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