International Journal of Insect Science

Efficacy of the combined treatment of a neem oil formulation and endosulfan on feeding and midgut enzyme activities of Helicoverpa ­armigera larvae was studied. The antifeedant activity was recorded at 24 h after treatment and the activities of midgut digestive (total serine protease and trypsin) and detoxifying (esterase and glutathione-S-transferase) enzymes were estimated at 72 h after treatment. The antifeedant activity in ­endosulfan + neem oil formulation (endosulfan 0.01% and neem oil formulation 1% at 1:1 ratio) was 85.34%, significantly greater than in individual treatments. Midgut digestive enzymes and EST activities were significantly reduced and the GST activity significantly increased in the combined treatment of endosulfan + neem oil formulation, thus showing increased effect of the combined treatment of the two pesticides. These results suggest that neem oil can be used in combination with endosulfan to reduce its quantity.