Publication Date: 17 Aug 2009
Type: Original Research
Journal: Microbiology Insights
Citation: Microbiology Insights 2009:2 39-49
Proteinase-K resistant prion protein (PrPres) has the property to aggregate in TSE-injured animal tissues. We have developed a test method to discriminate scrapie-infected and mock-infected hamsters by detecting the PrPres in plasma. It seemed that aggregation of the PrPres with some heterogeneous molecule(s) enabled successful detection by this method. In order to investigate which molecule became the partner in the PrPres aggregates; we examined some molecules that could presumably have this ability. As a result, we found synthetic Poly-A RNA, especially in its denatured form, to be the most effective entity although glycoprotein, sulfated polysaccharide showed less effectiveness. DNA in the denatured form also has a high affinity, although in the presence of protein the effectiveness unsuccessful. On the basis of this result, it is possible that the PrPres aggregate in scrapie-infected hamster plasma is composed of PrPres and RNA.
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We made our first submission to Microbiology Insights. We are very impressed by the helpful guidance during paper submission, the prompt processing (less than two weeks to editorial decision), and the flexibility of the editor-in-chief. We believe that Microbiology Insights journal is a unique platform that offer a high visibility for microbiologists, particularly in resource-poor settings.
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