Close
Help




JOURNAL

Gene Regulation and Systems Biology

Role of SP-1 in SDS-Induced Adipose Differentiation Related Protein Synthesis in Human Keratinocytes

Submit a Paper


Gene Regulation and Systems Biology 2007:1 207-215

Published on 04 Oct 2007


Further metadata provided in PDF



Sign up for email alerts to receive notifications of new articles published in Gene Regulation and Systems Biology

Emanuela Corsini, Omar Zancanella, Laura Lucchi, Barbara Viviani, Marina Marinovich and Corrado L. Galli

Laboratory of Toxicology, Department of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milan, Italy.

Abstract: Skin irritation is a complex phenomenon, and keratinocytes play an important role in it. We have recently characterized the expression and protective role of adipose differentiation related protein (ADRP) in skin irritation. In particular, ADRP expression is induced to recover functional cell membrane following the cell damage caused by skin irritants. The purpose of this study was to characterize in a human keratinocyte cells line (NCTC 2544) the biochemical events that lead to ADRP expression following SDS treatment, and in particular, to investigate the role of transcription factor SP-1. Analysis of ADRP promoter region revealed the presence of a potential binding site for the transcription factor SP-1 close to the start site. Evaluated by measuring the DNA binding activity, we found that SDS induced a dose and time related SP-1 activation, which was correlated with SDS-induced ADRP mRNA expression. Furthermore, SDS-induced SP-1 activation, ADRP mRNA expression and lipid droplets accumulation could be modulated by mithramycin A, an antibiotic that selectively binds to the GC box preventing SP-1 binding and gene expression. This demonstrated that SDS-induced ADRP expression was mediated in part through the transcription factor SP-1. In addition, SDS-induced SP-1 activation and ADRP expression could be modulated by the calcium chelator BAPTA, indicating a role of calcium in ADRP-induction. Thus, every time an irritant perturbs the membrane barrier, it renders the membrane leaky and allows extracellular calcium to enter the cells, an event that provides the upstream mechanisms initiating the signaling cascade that triggers the activation of SP-1 and culminates in the enhancement of ADRP expression, which helps to restore the normal homeostasis and ultimately repairs the to membrane.



Downloads

PDF  (494.78 KB PDF FORMAT)

RIS citation   (ENDNOTE, REFERENCE MANAGER, PROCITE, REFWORKS)

BibTex citation   (BIBDESK, LATEX)

XML

PMC HTML


Sharing


What Your Colleagues Say About Gene Regulation and Systems Biology
Since my first enquiry about publishing in Gene Regulation And Systems Biology until the last moment of completing all the steps for publishing my paper, I was always taken seriously as author.  All my questions and concerns were answered in a very professional way.  The review process was quick and very fair.  Reviewers stick to the facts and declare their points of view like a clear thread through the manuscript.  I always had an enthusiastic ...
Dr Jasmine Kharazmi (University of Zurich, Irchel, Zurich, Switzerland)
More Testimonials

Quick Links


New article and journal news notification services
Email Alerts RSS Feeds
Facebook Google+ Twitter
Pinterest Tumblr YouTube