Publication Date: 19 Oct 2010
Type: Original Research
Journal: Gene Regulation and Systems Biology
Citation: Gene Regulation and Systems Biology 2010:4 91-101
In contrast to rodents, adipose tissue serves as the major site of lipogenesis and storage reservoir for excess dietary energy in cattle. Research in rodents shows that adding corn oil (57% C18:2 n-6) to the diet alters lipogenesis enhancing deposition of omega-6 fatty acids. This study examines changes in lipogenic gene expression of subcutaneous adipose tissue from eighteen steers fed increasing levels of dietary corn oil [0 (NONE), 0.31 kg/d (MED) and 0.62 kg/d (HI)] using two platforms, qPCR and microarray. The results show that MED level of oil supplementation up-regulates gene expression of key lipogenic enzymes but that as oil supplementation reaches HI level mRNA encoding lipogenic enzymes responsible for de novo synthesis and desaturation are down-regulated. Changes in specific lipogenic mRNA levels are correlated with changes in tissue fatty acid composition where de novo and desatured fatty acids were reduced with the highest level of oil supplementation.
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Since my first enquiry about publishing in Gene Regulation And Systems Biology until the last moment of completing all the steps for publishing my paper, I was always taken seriously as author. All my questions and concerns were answered in a very professional way. The review process was quick and very fair. Reviewers stick to the facts and declare their points of view like a clear thread through the manuscript. I always had an enthusiastic ...
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