Help
Posted Wed, Apr, 10,2013
Published today in Microbiology Insights is a new original research article by Rafidah Mohd Ariff, Anwar Fitrianto, Mohd Yazid Abd. Manap, Aini Ideris, Azhar Kassim, Afinah Suhairin and Anis Shobirin Meor Hussin. Read more about this paper below:
Title
Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α
Abstract
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
Click here to learn more about the article, download it and comment
Posted in: Articles Published
News Categories
Thu 08 Oct, 2015
Published This Week (5th - 9th October)Thu 08 Oct, 2015
Biomarker Insights Paper Endorsed by Editor in ChiefWed 07 Oct, 2015
Interview with Professor Jamie DaviesI found the publication process for Human Parasitic Diseases very smooth and helpful. Particularly, I was impressed about the stage-notifications that I received. The review and editorial processes were exceptionally fast. The reviewer comments were constructive and of high quality. An excellent job done by the entire team.
Facebook Google+ Twitter
Pinterest Tumblr YouTube