Publication Date: 15 May 2013
Type: Original Research
Journal: Gene Regulation and Systems Biology
Citation: Gene Regulation and Systems Biology 2013:7 85-102
Products of the myc gene family integrate extracellular signals by modulating a wide range of their targets involved in cellular biogenesis and metabolism; the purpose of this integration is to regulate cell death, proliferation, and differentiation. However, understanding the regulation of myc at the transcription level remains a challenge. We performed rapid amplification of dmyc cDNA ends (5′ RACE) and mapped the transcription start site at P1 promoter, 18 base pairs upstream of the start of the known EST GM01143 and within the 5′ UTR. Our data show that the first TATA box, previously computationally predicted, is utilized to generate dmyc full length mRNA. The largest transcript contains all three exons, generated after the removal of the introns by constitutively regulated splicing events. Further investigation of Downstream Promoter Element (DPE) was achieved by studying lacZ reporter activity; investigation revealed that this element and its upstream cluster of binding sites are required for the dmyc intron 2 activity. These findings may provide valuable tools for further analysis of dmyc cis-elements.
PDF (1.74 MB PDF FORMAT)
RIS citation (ENDNOTE, REFERENCE MANAGER, PROCITE, REFWORKS)
BibTex citation (BIBDESK, LATEX)
Since my first enquiry about publishing in Gene Regulation And Systems Biology until the last moment of completing all the steps for publishing my paper, I was always taken seriously as author. All my questions and concerns were answered in a very professional way. The review process was quick and very fair. Reviewers stick to the facts and declare their points of view like a clear thread through the manuscript. I always had an enthusiastic ...
Facebook Google+ Twitter
Pinterest Tumblr YouTube