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Protein–protein interactions play a pivotal role in both inter- and intra-cellular signaling. Identification of signaling protein complexes can thus shed important new insights into cell communications. We developed a parallel affinity precipitation protocol to overcome the disadvantages of the tandem affinity purification procedure, such as the potential disruption of target protein conformation, subcellular localization or function by epitope tags, the potential need of large amounts of cell culture or generation of stable cell lines, and relatively long duration the two-step precipitation takes. This new simplified assay of protein interaction is quick, economic and specific. This paper describes the details in the design and method of the assay.
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My laboratory has published several papers in Cell Communication Insights. In each case, reviewer comments were returned promptly; the suggested revisions were both fair and quite helpful, reflecting positively on the quality of the review. Once accepted, the editorial office provided clear and frequent updates on the progress of our manuscripts through each step of the publication process. When necessary, I had prompt email responses to my questions and the figure quality was exceptional. Keep ...
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