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Virology: Research and Treatment

Synopsis: An open access, peer reviewed electronic journal that covers all aspects of virus research and treatment in humans, animals, plants, bacteria and fungi.


Indexing: 5 major databases. Pubmed indexing for NIH-funded research.

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About this journal

ISSN: 1178-122X



Aims and scope:

Virology: Research and Treatment is an international, open access, peer reviewed journal which considers manuscripts on all aspects of viruses. All articles on human, animal, insect, plant, and bacterial and fungal viruses are considered.

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Submissions, excluding editorials, letters to the editor and dedications, will be peer reviewed by two reviewers.  Reviewers are required to provide fair, balanced and constructive reports.  

Under our Fairness in Peer Review Policy authors may appeal against reviewers' recommendations which are ill-founded, unobjective or unfair.  Appeals are considered by the Editor in Chief or Associate Editor.

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As of April 7 2008, the US NIH Public Access Policy requires that all peer reviewed articles resulting from research carried out with NIH funding be deposited in the Pubmed Central archive.

If you are an NIH employee or grantee Libertas Academica will ensure that you comply with the policy by depositing your paper at Pubmed Central on your behalf. 



 
 
 


Identification of Rotavirus VP6-Specific CD4+ T Cell Epitopes in a G1P[8] Human Rotavirus-Infected Rhesus Macaque

Authors: Wei Zhao, Bapi Pahar and Karol Sestak
Publication Date: 03 Mar 2008
Virology: Research and Treatment 2008:1 9-15

Wei Zhao1, Bapi Pahar1,2 and Karol Sestak1,2

1Tulane National Primate Research Center, Covington, LA, U.S.A. 2Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, U.S.A.

Abstract

A non-human primate model was used to evaluate its potential for identification of rotavirus viral protein 6 (VP6) CD4+ T cell epitopes. Four juvenile rhesus macaques were inoculated with a mixed inoculum (G1P[8] and G9P[8]) of human rotaviruses. Infection accompanied by G1P[8] shedding was achieved in the two macaques that had no rotavirus immunoglobulin A (IgA) in plasma. To measure the interferon gamma (IFN-γ) and tumor necrosis factor (TNF) anti-viral cytokines produced by peripheral CD4+ cells that recognize VP6 epitopes, whole blood cells from one infected macaque were stimulated in vitro with VP6 peptides. Stimulation with peptide pools derived from the simian rotavirus VP6161–395 region revealed reactivity of CD4+ T cells with the VP6281–331 domain. A VP6301–315 region was identified as the epitope responsible for IFN-γ production while a broader VP6293–327 domain was linked to TNF production. These results suggest that human rotavirus-infected macaques can be used for identification of additional epitopes and domains to address specific questions related to the development of pediatric vaccines.

Categories: Virology


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