Detection and Quantification of Plasmodium DNA in Dried Blood Spots Using a Commercial Real-Time PCR Assay and Filter Card System

Thomas Hanscheid; Vera Codices; Adrian J.F. Luty; Ayola A. Adegnika; Peter G. Kremsner; Martin P. Grobusch

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Human Parasitic Diseases

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Detection and Quantification of Plasmodium DNA in Dried Blood Spots Using a Commercial Real-Time PCR Assay and Filter Card System

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Authors: Thomas Hanscheid, Vera Codices, Adrian J.F. Luty, Ayola A. Adegnika, Peter G. Kremsner and Martin P. Grobusch

Publication Date: 03 Feb 2009

Journal: Human Parasitic Diseases

Citation: Human Parasitic Diseases 2009:1 1-4

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Abstract

Blood collected on FTA® filter cards was analysed qualitatively and quantitatively with real-time PCR and the results compared with expert microscopy performed on-site in Gabon. There was 100% concordance for fresh blood samples. However, correlation of quantitative PCR-results was rather weak (0.52) and reproducibility showed coefficients of variation ranging from 10%–90%.

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