Posted Wed, Apr, 17,2013
Published today in Analytical Chemistry Insights is a new original research article by Asmaa A. El-Zaher and Marianne A. Mahrouse. Read more about this paper below:
Title
Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human PlasmaAbstract
A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the derivatization step. The resulting highly fluorescent stable derivative was chromatographed on C18 column and eluted using methanol:water (60:40, v/v) at a flow rate of 1 mL min-1. A fluorescence detector (λex 390 and λem 480 nm) was used. The method was linear over the concentration range 20–200 ng mL-1. The structure of the fluorescent product was proposed, the method was then validated and applied for the determination of TOB in human plasma. The results were statistically compared with the reference method, revealing no significant difference.
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Thu 08 Oct, 2015
Published This Week (5th - 9th October)Thu 08 Oct, 2015
Biomarker Insights Paper Endorsed by Editor in ChiefWed 07 Oct, 2015
Interview with Professor Jamie DaviesIt was of great opportunity to publish my work with Libertas Academica. It was a truly pleasant experience. The entire process was rapid, very efficient and author friendly. I appreciate the kind, effective and helpful comments given by the peer reviewers. I am impressed with the high quality of service provided by Libertas Academica throughout this entire editorial process. I sincerely recommend to authors to publish their work and research with Libertas Academica. ...
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